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Part:BBa_J99002:Design

Designed by: David Bikard   Group: Mazel Lab   (2009-12-17)

attI1 recombination site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplified from p929 with oligos 5'-AAAGAATTCGCGGCCGCTTCTAGATGATGTTATGGAGCAGCAACGATGTT-3' and 5'-AGCCTGCAGCGGCCGCTACTAGTAATGTGATGCCTAACTTTGTTTTAGGGCG-3'


Source

p929 (Biskri et al., 2005)

References

Biskri, L. et al. Comparative study of class 1 integron and Vibrio cholerae superintegron integrase activities. Journal of bacteriology 187, 1740-50(2005).

Partridge, S. et al. Definition of the attI1 site of class 1 integrons. Microbiology (Reading, England) 146 ( Pt 1, 2855-2864(2000).